amitriptyline hcl Search Results


1217 2010 1266 1272 laudanosine  (LGC Standards)
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LGC Standards 1217 2010 1266 1272 laudanosine
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cyclobenzaprine  (Millipore)
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Millipore cyclobenzaprine
Linearity of swab-based recovery on metal surface at 45 min and 1 week for (A) carnitine, (B) phenylacetylglutamine and (C) <t>cyclobenzaprine.</t> Error bars represent standard error.
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c 120 duloxetine chem scene cat  (Chem Impex International)
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Chem Impex International c 120 duloxetine chem scene cat
Linearity of swab-based recovery on metal surface at 45 min and 1 week for (A) carnitine, (B) phenylacetylglutamine and (C) <t>cyclobenzaprine.</t> Error bars represent standard error.
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amitriptyline hcl [an inhibitor of the acid sphingomyelinase (smase)]  (GlpBio Technology Inc)
90
GlpBio Technology Inc amitriptyline hcl [an inhibitor of the acid sphingomyelinase (smase)]
Downregulation of ceramide levels promotes PoRV replication. (A) Schematic representation of ceramide formation pathways and respective inhibitors. (B and F) IPEC-J2 cells were treated with the inhibitors at the indicated concentrations for 24 h. CCK8 assay was used to evaluate their cytotoxicity on IPEC-J2 cells. (C and G) IPEC-J2 cells were treated with the inhibitors for 12 h; then, all cells were collected for total lipid extraction. LC-MS analysis was applied to measure the abundance of C24 in cells compared with in untreated cells. (D–E and H–I) IPEC-J2 cells pretreated with Fumonisin B (50 µM), Myriocin (20 µM), GW4869 (10 µM), or <t>Amitriptyline</t> (30 µM) for 12 h and then infected with PoRV (0.1 MOI) for 6 h. Cells and supernatants were harvested for analysis of the relative levels of PoRV NSP5 mRNA (D and H) and viral titers, respectively (E and I). The data were generated from three independent experiments (means ± SD). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.
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amitriptyline hcl cf tablet  (Centrafarm Nederland B V)
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Downregulation of ceramide levels promotes PoRV replication. (A) Schematic representation of ceramide formation pathways and respective inhibitors. (B and F) IPEC-J2 cells were treated with the inhibitors at the indicated concentrations for 24 h. CCK8 assay was used to evaluate their cytotoxicity on IPEC-J2 cells. (C and G) IPEC-J2 cells were treated with the inhibitors for 12 h; then, all cells were collected for total lipid extraction. LC-MS analysis was applied to measure the abundance of C24 in cells compared with in untreated cells. (D–E and H–I) IPEC-J2 cells pretreated with Fumonisin B (50 µM), Myriocin (20 µM), GW4869 (10 µM), or <t>Amitriptyline</t> (30 µM) for 12 h and then infected with PoRV (0.1 MOI) for 6 h. Cells and supernatants were harvested for analysis of the relative levels of PoRV NSP5 mRNA (D and H) and viral titers, respectively (E and I). The data were generated from three independent experiments (means ± SD). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.
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nortriptyline  (Cerilliant Corporation)
90
Cerilliant Corporation nortriptyline
Downregulation of ceramide levels promotes PoRV replication. (A) Schematic representation of ceramide formation pathways and respective inhibitors. (B and F) IPEC-J2 cells were treated with the inhibitors at the indicated concentrations for 24 h. CCK8 assay was used to evaluate their cytotoxicity on IPEC-J2 cells. (C and G) IPEC-J2 cells were treated with the inhibitors for 12 h; then, all cells were collected for total lipid extraction. LC-MS analysis was applied to measure the abundance of C24 in cells compared with in untreated cells. (D–E and H–I) IPEC-J2 cells pretreated with Fumonisin B (50 µM), Myriocin (20 µM), GW4869 (10 µM), or <t>Amitriptyline</t> (30 µM) for 12 h and then infected with PoRV (0.1 MOI) for 6 h. Cells and supernatants were harvested for analysis of the relative levels of PoRV NSP5 mRNA (D and H) and viral titers, respectively (E and I). The data were generated from three independent experiments (means ± SD). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.
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amitriptyline  (Selleck Chemicals)
92
Selleck Chemicals amitriptyline
Downregulation of ceramide levels promotes PoRV replication. (A) Schematic representation of ceramide formation pathways and respective inhibitors. (B and F) IPEC-J2 cells were treated with the inhibitors at the indicated concentrations for 24 h. CCK8 assay was used to evaluate their cytotoxicity on IPEC-J2 cells. (C and G) IPEC-J2 cells were treated with the inhibitors for 12 h; then, all cells were collected for total lipid extraction. LC-MS analysis was applied to measure the abundance of C24 in cells compared with in untreated cells. (D–E and H–I) IPEC-J2 cells pretreated with Fumonisin B (50 µM), Myriocin (20 µM), GW4869 (10 µM), or <t>Amitriptyline</t> (30 µM) for 12 h and then infected with PoRV (0.1 MOI) for 6 h. Cells and supernatants were harvested for analysis of the relative levels of PoRV NSP5 mRNA (D and H) and viral titers, respectively (E and I). The data were generated from three independent experiments (means ± SD). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.
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amitriptyline l hcl  (Yamanouchi Pharmaceutical Co)
90
Yamanouchi Pharmaceutical Co amitriptyline l hcl
Downregulation of ceramide levels promotes PoRV replication. (A) Schematic representation of ceramide formation pathways and respective inhibitors. (B and F) IPEC-J2 cells were treated with the inhibitors at the indicated concentrations for 24 h. CCK8 assay was used to evaluate their cytotoxicity on IPEC-J2 cells. (C and G) IPEC-J2 cells were treated with the inhibitors for 12 h; then, all cells were collected for total lipid extraction. LC-MS analysis was applied to measure the abundance of C24 in cells compared with in untreated cells. (D–E and H–I) IPEC-J2 cells pretreated with Fumonisin B (50 µM), Myriocin (20 µM), GW4869 (10 µM), or <t>Amitriptyline</t> (30 µM) for 12 h and then infected with PoRV (0.1 MOI) for 6 h. Cells and supernatants were harvested for analysis of the relative levels of PoRV NSP5 mRNA (D and H) and viral titers, respectively (E and I). The data were generated from three independent experiments (means ± SD). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.
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amitriptyline-d6 hcl (n,n-dimethyl-d6)  (Qmx Laboratories Ltd)
90
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Downregulation of ceramide levels promotes PoRV replication. (A) Schematic representation of ceramide formation pathways and respective inhibitors. (B and F) IPEC-J2 cells were treated with the inhibitors at the indicated concentrations for 24 h. CCK8 assay was used to evaluate their cytotoxicity on IPEC-J2 cells. (C and G) IPEC-J2 cells were treated with the inhibitors for 12 h; then, all cells were collected for total lipid extraction. LC-MS analysis was applied to measure the abundance of C24 in cells compared with in untreated cells. (D–E and H–I) IPEC-J2 cells pretreated with Fumonisin B (50 µM), Myriocin (20 µM), GW4869 (10 µM), or <t>Amitriptyline</t> (30 µM) for 12 h and then infected with PoRV (0.1 MOI) for 6 h. Cells and supernatants were harvested for analysis of the relative levels of PoRV NSP5 mRNA (D and H) and viral titers, respectively (E and I). The data were generated from three independent experiments (means ± SD). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.
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amitriptyline hydrochloride amt-hcl  (BLDpharm)
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BLDpharm amitriptyline hydrochloride amt-hcl
Downregulation of ceramide levels promotes PoRV replication. (A) Schematic representation of ceramide formation pathways and respective inhibitors. (B and F) IPEC-J2 cells were treated with the inhibitors at the indicated concentrations for 24 h. CCK8 assay was used to evaluate their cytotoxicity on IPEC-J2 cells. (C and G) IPEC-J2 cells were treated with the inhibitors for 12 h; then, all cells were collected for total lipid extraction. LC-MS analysis was applied to measure the abundance of C24 in cells compared with in untreated cells. (D–E and H–I) IPEC-J2 cells pretreated with Fumonisin B (50 µM), Myriocin (20 µM), GW4869 (10 µM), or <t>Amitriptyline</t> (30 µM) for 12 h and then infected with PoRV (0.1 MOI) for 6 h. Cells and supernatants were harvested for analysis of the relative levels of PoRV NSP5 mRNA (D and H) and viral titers, respectively (E and I). The data were generated from three independent experiments (means ± SD). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.
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amitriptyline hcl  (Hexal AG)
90
Hexal AG amitriptyline hcl
Downregulation of ceramide levels promotes PoRV replication. (A) Schematic representation of ceramide formation pathways and respective inhibitors. (B and F) IPEC-J2 cells were treated with the inhibitors at the indicated concentrations for 24 h. CCK8 assay was used to evaluate their cytotoxicity on IPEC-J2 cells. (C and G) IPEC-J2 cells were treated with the inhibitors for 12 h; then, all cells were collected for total lipid extraction. LC-MS analysis was applied to measure the abundance of C24 in cells compared with in untreated cells. (D–E and H–I) IPEC-J2 cells pretreated with Fumonisin B (50 µM), Myriocin (20 µM), GW4869 (10 µM), or <t>Amitriptyline</t> (30 µM) for 12 h and then infected with PoRV (0.1 MOI) for 6 h. Cells and supernatants were harvested for analysis of the relative levels of PoRV NSP5 mRNA (D and H) and viral titers, respectively (E and I). The data were generated from three independent experiments (means ± SD). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.
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the isotopic internal standards (is), amitriptyline-d6 hcl (ami-d6)  (Qmx Laboratories Ltd)
90
Qmx Laboratories Ltd the isotopic internal standards (is), amitriptyline-d6 hcl (ami-d6)
Downregulation of ceramide levels promotes PoRV replication. (A) Schematic representation of ceramide formation pathways and respective inhibitors. (B and F) IPEC-J2 cells were treated with the inhibitors at the indicated concentrations for 24 h. CCK8 assay was used to evaluate their cytotoxicity on IPEC-J2 cells. (C and G) IPEC-J2 cells were treated with the inhibitors for 12 h; then, all cells were collected for total lipid extraction. LC-MS analysis was applied to measure the abundance of C24 in cells compared with in untreated cells. (D–E and H–I) IPEC-J2 cells pretreated with Fumonisin B (50 µM), Myriocin (20 µM), GW4869 (10 µM), or <t>Amitriptyline</t> (30 µM) for 12 h and then infected with PoRV (0.1 MOI) for 6 h. Cells and supernatants were harvested for analysis of the relative levels of PoRV NSP5 mRNA (D and H) and viral titers, respectively (E and I). The data were generated from three independent experiments (means ± SD). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.
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Image Search Results


Linearity of swab-based recovery on metal surface at 45 min and 1 week for (A) carnitine, (B) phenylacetylglutamine and (C) cyclobenzaprine. Error bars represent standard error.

Journal: bioRxiv

Article Title: Optimized extraction method enables quantitative analysis of surface metabolite recovery for exposomics and behavioral studies

doi: 10.1101/2021.08.25.457715

Figure Lengend Snippet: Linearity of swab-based recovery on metal surface at 45 min and 1 week for (A) carnitine, (B) phenylacetylglutamine and (C) cyclobenzaprine. Error bars represent standard error.

Article Snippet: Carnitine (VWR), cyclobenzaprine (Cerilliant), erythromycin (Sigma), oleanolic acid (Cayman) and phenylacetylglutamine (Cayman) were prepared in 50% methanol and stored at 4°C until usage.

Techniques:

Downregulation of ceramide levels promotes PoRV replication. (A) Schematic representation of ceramide formation pathways and respective inhibitors. (B and F) IPEC-J2 cells were treated with the inhibitors at the indicated concentrations for 24 h. CCK8 assay was used to evaluate their cytotoxicity on IPEC-J2 cells. (C and G) IPEC-J2 cells were treated with the inhibitors for 12 h; then, all cells were collected for total lipid extraction. LC-MS analysis was applied to measure the abundance of C24 in cells compared with in untreated cells. (D–E and H–I) IPEC-J2 cells pretreated with Fumonisin B (50 µM), Myriocin (20 µM), GW4869 (10 µM), or Amitriptyline (30 µM) for 12 h and then infected with PoRV (0.1 MOI) for 6 h. Cells and supernatants were harvested for analysis of the relative levels of PoRV NSP5 mRNA (D and H) and viral titers, respectively (E and I). The data were generated from three independent experiments (means ± SD). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.

Journal: Journal of Virology

Article Title: Lipidomics reveals the significance and mechanism of the cellular ceramide metabolism for rotavirus replication

doi: 10.1128/jvi.00064-24

Figure Lengend Snippet: Downregulation of ceramide levels promotes PoRV replication. (A) Schematic representation of ceramide formation pathways and respective inhibitors. (B and F) IPEC-J2 cells were treated with the inhibitors at the indicated concentrations for 24 h. CCK8 assay was used to evaluate their cytotoxicity on IPEC-J2 cells. (C and G) IPEC-J2 cells were treated with the inhibitors for 12 h; then, all cells were collected for total lipid extraction. LC-MS analysis was applied to measure the abundance of C24 in cells compared with in untreated cells. (D–E and H–I) IPEC-J2 cells pretreated with Fumonisin B (50 µM), Myriocin (20 µM), GW4869 (10 µM), or Amitriptyline (30 µM) for 12 h and then infected with PoRV (0.1 MOI) for 6 h. Cells and supernatants were harvested for analysis of the relative levels of PoRV NSP5 mRNA (D and H) and viral titers, respectively (E and I). The data were generated from three independent experiments (means ± SD). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.

Article Snippet: Myriocin [a potent inhibitor of serine palmitoyltransferase (SPT) served as the rate-limiting enzyme in the de novo pathway], Fumonisin B1 (an inhibitor of ceramide synthases in both the de novo and salvage pathways), Amitriptyline HCl [an inhibitor of the acid sphingomyelinase (SMase)], GW4869 (an inhibitor of the neutral SMase), and Z-VAD-FMK (an inhibitor of apoptosis) were obtained from GLPBIO (Montclair, CA, USA) and dissolved in sterile dimethyl sulfoxide at concentrations ranging from 5 mM to 20 mM ( 43 , – 46 ).

Techniques: CCK-8 Assay, Extraction, Liquid Chromatography with Mass Spectroscopy, Infection, Generated